Changes between Version 7 and Version 8 of SNPPipelineTestManual
- Timestamp:
- Dec 23, 2010 3:19:50 PM (14 years ago)
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SNPPipelineTestManual
v7 v8 16 16 * GATK 17 17 - The GATK has several dependancies: 18 - A nt18 - Apache Ant 19 19 - JRE 20 - Make sure you download the GATK version from the SVN, since this version contains scripts which aren't available in the pre-build version 20 21 - More information to be found here: http://www.broadinstitute.org/gsa/wiki/index.php/Prerequisites & http://www.broadinstitute.org/gsa/wiki/index.php/Downloading_the_GATK 21 22 * SAMtools 22 23 * Pindel 23 24 * FastQC 25 26 Other less important tools: 27 * To run GATK variant eval using Rscript the following steps need to be executed: 28 - 1. Add the path to GATK R directory to your .Rprofile (usually this file is in your home directory, otherwise just create it). 29 - Open your .Rprofile file and add .libPaths(“/path/to/Sting/R”) to the file 30 - 2. If you haven't set the path to your R installation execute: 31 - PATH=$PATH:/path/to/R/bin 32 - 3.Go to GATK directory, in this case /path/to/Sting 33 - execute the following command: ant gsalib 24 34 25 35 '''__Step 3__''' Configure pipeline