Groningen cluster

People UMCG: Morris, Freerk, more?

Description Description here about code template and automatic PBS script generation. Job submission/monitoring

Port applications to Dutch Life Science Grid

People

• AMC: Antoine van Kampen, Barbera van Schaik, Silvia D Olabarriaga, Mark Santcroos
• Sara/BiGGrid: Tom Visser, more?
• UMCG: Morris, Freerk

Description Software is going to be implemented as workflow components. The workflows will run on the Dutch life science grid.

• Information about the infrastructure: ​http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/
• Getting started: ​http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/GettingStarted

Implemented workflow components at AMC

This list of workflow components are already available. We can expand it with Pindel and (parts of) the GATK pipeline.

• Splitting of fastq files
• Building a BWA index on the genome sequence (base space and color space)
• BWA for shotgun reads (base space and color space) It is possible to do parameter sweeps. Output is in bam format
• Merge bam results
• Samtools pileup
• Varscan (pileup to snp, indel and cns)
• Bam2coverage creates a UCSC wiggle file to display the genome coverage (per 50kbp)
• Coverage-per-base determines the coverage for every base in the genome and it summarizes the results (coverage versus frequency)
• Annovar (works for hg18, working on other assemblies). This is a pipeline to annotate variants (gene, dbsnp, hapmap, 1000g, conservation, etc)
• FastqC

Implemented components of the Groningen pipeline A more detailed description will follow later

• BwaIllumina (done) - pe00-bwa-align-pair1.ftl, pe01-bwa-align-pair2.ftl, pe02-bwa-sampe.ftl, pe03-sam-to-bam.ftl, pe04-sam-sort.ftl
• MarkDuplicates (done) - pe05-mark-duplicates.ftl
• PicardQC (partly done) - pe04b-picardQC.ftl. Didn't get the R environment up and running yet, so the .pdf .hist and .bamindexstats can not be produced yet. Will continue with the other components and fix this later. Attachment contains info about the required R packages.
• GatkGenerateIntervalFile (done) - see e-mail Freerk on Dec 13, 2010
• ReAlign (done) - pe06-realign.ftl

To be implemented

• The components of the Groningen pipeline that not implemented as a workflow component yet
• Pindel

Data access rights

To ensure that the most limited group of people has access to the data we have created a subgroup "gvnl" within the "vlemed" Virtual Organisation (VO). For people to become part of this group, it is required that they have a Grid certificate and that they are part of the "vlemed" VO. On the following page there is information on how to get a certificate, how to get into the "vlemed" VO: ​http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/EBioInfra#Access

For more information about data access see ​http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/DataManagement

Things to address

• Available disk space on the grid storage elements / worker nodes

Data location on grid

Data

Browse to the following directory with the Vbrowser (this directory is only readable/writable for the gvnl group): lfn://lfc.grid.sara.nl:5010/grid/vlemed/gvnl/Data

Each component in the workflow generates a new sub directory.

If you would like to retrieve the analysis results of the picard-qc component of one of the samples, you go to the directory:

• bwa_databasename_parametername/samplename_libraryname_lanename/picard1.32qc/
• Example: lfn://lfc.grid.sara.nl:5010/grid/vlemed/gvnl/Data/bwa_gatk_index_basespace_human_g1k_v37.fasta.tar.gz_bwa_4_threads.txt/A4a_HUModqRBVDIBAPE_L7

The results for the analysis where we have splitted the fastq-files is located in the directories that start with:

• split_fileprefix_1/

Note that not everything is there yet and that the data structure may change, since we are still testing.

Workflows and databases

These directories are open to all members of the vlemed VO

• Workflows: lfn://lfc.grid.sara.nl:5010/grid/vlemed/AMC-e-BioScience/Sequence_WF
• Databases: lfn://lfc.grid.sara.nl:5010/grid/vlemed/AMC-e-BioScience/Sequence_DB

The directories that contain the workflows have the following structure:

Directory	Description
bin	dependent binaries like bwa, samtools
GasW	component description, describes which executable has to run on grid and it specifies the in and output files/parameters
Java	dependent jar files, e.g. the GATK jar
parameterFiles	text files that contain exactly one line with the parameters that you would like to provide to bwa or another component
Scufl	old workflow description files, can be ignored
shFiles	these files are executed on the grid and described by the GASW descriptor, the gvnl shFiles are based on the Groningen templates
Workflows	Workflow descriptions, clicking on them will start the Moteur plugin. Input files/parameters can be specified in the fields. If you click on "Run" the jobs are submitted to the grid

More info in ​Luyf and van Schaik et al (2010)

The shell files can (in most cases) run on any linux cluster. In that case you need to place the shell file and the dependent executable(s) in one directory. At the start of each shell file is an example on how to run them.

Workflow execution

On mini pilot. Split lines-per-file: 8,000,000. Start: 18-12-2010 16:40

Lane	split BWA merge	Comments	Elapsed time (s)
A4a_L4_HUModqRBUDIBAPE	​done		22740
A4a_L6_HUModqRADDIAAPE	​done		37750
A4a_L7_HUModqRBVDIBAPE	​failed
A4b_L3_HUModqRAFDIBAPE	​done		32256
A4b_L6_HUModqRBTDIBAPE	​failed
R2A _L1_HUModqRADDIBAPE	​failed
R2A _L1_HUModqRAFDIBAPE	​done		35819
R2A _L5_HUModqRAEDIAAPE	​failed
R2B _L3_HUModqRBTDIAAPE	​done		23754
R2B _L4_HUModqRBUDIAAPE	​failed	pair 1 not in correct gzip format?
R2B _L6_HUModqRBTDIBAPE	​failed
R2C _L2_HUModqRBUDIBAPE	​done		40763
R2C _L2_HUModqRBVDIBAPE	​failed
R2C _L7_HUModqRBVDIAAPE	​done		39374
Unknown_L6_HUModqRBUDIAAPE	​done		30307

Note: The Merge component is red (marked as failed) in the finished workflows. The bam files where produced successfully.

BWA alignment on mini pilot without splitting the data

• 19-12-2010 14:00 ​running

PicardQC on finished bam files

• 20-12-2010 11:30 ​done - elapsed time (10 bam files) 8730 s
• ​Info runtime and used disk space (ods)

Coverage-per-base on finished bam files

• 20-12-2010 12:40 ​running

Mark-duplicates on finished bam files

• 20-12-2010 12:47 ​done - elapsed time 10614 s

Re-align on finished bam files

• 20-12-2010 17:48 ​done - elapsed time 9165 s

Gvnl pipeline on remaining samples (split, bwa, merge, coverage-per-base, picard-qc, mark-duplicates, re-align) Start: 20-12-2010 18:50

Lane	Progress
A4a_L7_HUModqRBVDIBAPE	​running
A4b_L6_HUModqRBTDIBAPE	​running
R2A _L1_HUModqRADDIBAPE	​running
R2A _L5_HUModqRAEDIAAPE	​done
R2B _L4_HUModqRBUDIAAPE	​failed
R2B _L6_HUModqRBTDIBAPE	​running
R2C _L2_HUModqRBVDIBAPE	​done

Note: Workflow stops after merge-bam

Alternatives

Clusters

• Groningen
• Leiden
• Huygens
• Lisa
• Philips
• DAS

Grid

• EBioInfra ​http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/
• BiGGrid Cloud ​http://www.cloud.sara.nl/
• Topos ​https://grid.sara.nl/wiki/index.php/Using_the_Grid/ToPoS